Cloning of a BDV-NS3 Conserved Fragment for the Preparation of a Reporter Cell Line

: Border disease virus (BDV) is a serious pathogen of sheep and goats worldwide. The causes considerable economic losses in animal husbandry. Therefore, developing effective genetic-based therapies to control viral infections, such as BDV, has been remarkable over recent years. A common way evaluate therapeutic strategies by cloning the desired fragment into appropriate vectors develop cell lines expressing it. Due required duties NS3 gene for BDV proliferation, this gene's HELICc nucleotide binding site domain was synthesized cloned lentiviral vector upstream GFP gene. accuracy verified digestion with restriction enzymes sequencing. Thus, BDV-NS3 carrying plasmid prepared successfully will be applied second or third packaging system stable expression